Oxidative stress is a fundamental biological process that plays a critical role in development, aging, and the progression of neurodegenerative diseases. Reactive oxygen species (ROS), such as hydrogen peroxide (H₂O₂), are naturally generated during cellular metabolism but can become cytotoxic when produced in excess. Neuronal cells are particularly vulnerable to oxidative damage due to their high metabolic demand and limited antioxidant capacity. The purpose of this study was to assess the effects of hydrogen peroxide-induced oxidative stress on N38 neuronal cells and to evaluate whether increasing concentrations of H₂O₂ result in decreased neuronal cell survival. N38 cells were cultured under standard conditions and exposed to increasing concentrations of hydrogen peroxide (0, 1, 10, and 100 µM). Following treatment, surviving cells were fixed, imaged, and quantified using ImageJ cell counting analysis. The results demonstrated a clear dose-dependent decrease in cell survival as hydrogen peroxide concentration increased, with the highest concentration causing near-complete cell loss. These findings support the hypothesis that hydrogen peroxide induces oxidative stress-mediated cytotoxicity in N38 neuronal cells. This study establishes a reproducible in vitro model of neuronal oxidative stress that can be applied to future investigations examining antioxidant-mediated neuroprotection and mechanisms of cellular aging.
- Assessment of Hydrogen Peroxide-Induced Oxidative Stress in mHypoE-N38 Cells
- Syed A BukhariAbdullah Sajid ChatthaBenjamin Weeks (Advisor) - Adelphi University, Biology
- Scholarship and Creative Works Conference (Adelphi University, 04/28/2026)
- Adelphi University; Biology
- Poster
- https://doi.org/10.48516/004525
- 991004536600006266